Human papillomavirus (HPV) type 16 and 18 have been strongly implicated as causative agents of uterine cervix carcinoma. However in Korea the association between HPV and uterine cervix carcinoma, and the exact prevalence of HPV have not been
clearly
documented. To study the relationship of HPV type 16/18 and cervical carcinoma in Korean women, we analyzed paraffin embedded tissues from 73 patients including 10 chronic cervicitis, 13 cervical intraepithelial neoplasia (CIN) and 50 carcinoma
(2
adenocarcinoma, 48 squamous cell carcinoma). 419 cervical scrapes from asymptomatic women showing normal cytology were also examined. The samples were analyzed by polymerase chain reaction. Cervical squamous cell carcinoma containing HPV type 16
DNA was
examined by electron microscopy to find probable virus particles.
In 419 cytologically normal scrapes, 101 (24.1%) were infected with HPV16/18. Of these. HPV 16, HPV 18 and HPV 16/18 revealed 19.1% (80/519), 4.5% (19/419), 0.5% (2/419) positive, respectively. HPV infection rate was the highest in 30-39 ages
group
(32.0%), followed by 40-49 ages group (26.1%). The prevalence of HPV 16/18 DNA declined with age in normal women. Of 10 CIN 0, HPV type 16 positive was 10% (1/10), but HPV type 18 DNA was not detected. Of 13 CIN grades, HPV 16 positive was 15.4%
(2/13),
and HPV type 18 was 7.7% (1/13).
There was no difference of HPV incidence among various degrees of CIN. Of 48 squamous cell carcinoma, HPV type 16 positive were 91.7% (44/48). HPV type 18 positive were 2.1% (1/48), and both HPV type 16 and 18 were 2.1% (1/48) positive. Infection
rate
of HPV 16/18 in carcinoma was higher than that of normal serapes and was the highest in ages group from 40 to 49. Virion particles were not detected on electron microscope.
However, the holo in cytoplasm, which is regarded as typical sign of HPV infection was observed. Relative risk associating HPV with cervix squamous carcinoma correlated markedly with HPV infection (p<0.001).
|